Characterization of Streptomyces venezuelae ATCC 10595 rRNA gene clusters and cloning of rrnA.

نویسندگان

  • M La Farina
  • S Stira
  • R Mancuso
  • C Grisanti
چکیده

Streptomyces venezuelae ATCC 10595 harbors seven rRNA gene clusters which can be distinguished by BglII digestion. The three rRNA genes present in each set are closely linked with the general structure 16S-23S-5S. We cloned rrnA and sequenced the 16S-23S spacer region and the region downstream of the 5S rRNA gene. No tRNA gene was found in these regions.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Complete Genome Sequence of Streptomyces venezuelae ATCC 15439, Producer of the Methymycin/Pikromycin Family of Macrolide Antibiotics, Using PacBio Technology

Here, we report the complete genome sequence of Streptomyces venezuelae ATCC 15439, a producer of the methymycin/pikromycin family of macrolide antibiotics and a model host for natural product studies, obtained exclusively using PacBio sequencing technology. The 9.03-Mbp genome harbors 8,775 genes and 11 polyketide and nonribosomal peptide natural product gene clusters.

متن کامل

Streptomyces sp. SCBT Isolated from Rhizosphere Soil of Medicinal Plants is Antagonistic to Pathogenic Bacteria

The Streptomycetes sp. SCBT strain Gen Bank accession number EU143270 isolated from the rhizosphere soil of medicinal plants was collected from the Kolli hills of Tamil Nadu, India. The strain Streptomyces is designated as School of Chemical and Biotechnology (SCBT), capable of inhibiting the growth of a wide range of Gram-negative and Gram-positive bacteria. An almost complete 16S rRNA gene se...

متن کامل

Streptomyces spp. as efficient expression system for a d,d-peptidase/d,d-carboxypeptidase involved in glycopeptide antibiotic resistance

BACKGROUND VanYn, encoded by the dbv7 gene (also known as vanYn) of the biosynthetic cluster devoted to A40926 production, is a novel protein involved in the mechanism of self-resistance in Nonomuraea sp. ATCC 39727. This filamentous actinomycete is an uncommon microorganism, difficult-to-handle but biotechnologically valuable since it produces the glycopeptide antibiotic A40926, which is the p...

متن کامل

Molecular cloning and characterization of an rRNA operon in Streptomyces lividans TK21.

The number of rRNA genes in Streptomyces lividans was examined by Southern hybridization. Randomly labeled 23 and 16S rRNAs were hybridized with BamHI, BglII, PstI, SalI, or XhoI digests of S. lividans TK21 DNA. BamHi, BglII, SalI and XhoI digests yielded six radioactive bands each for the 23 and 16S rRNAs, whereas PstI digests gave one band for the 23S rRNA and one high-intensity band and six ...

متن کامل

Isolation, Identification and Screening of Saharan Actinomycete Strain Streptomyces fimbriatus AC31 Endowed with Antimicrobial Activity

The increasing global public health concern of antimicrobial resistance (AMR) necessitates exploration of natural antimicrobial agents as potential alternatives. This study aims to investigate antimicrobial activities of Saharan actinomycetes, with specific focus on the strain Streptomyces fimbriatus AC31, that holds promising potential as an alternative to combat AMR. In this context, 32 actin...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of bacteriology

دوره 178 5  شماره 

صفحات  -

تاریخ انتشار 1996